03558nam a2200457Ii 4500 9780429458187 FlBoTFG 20260210180802.0 m o d cr 181112t20182019fluab ob 001 0 eng d 9780429458187 (e-book : PDF) (OCoLC)1049801226 FlBoTFG FlBoTFG rda eng QP551 MED 008000 bisacsh PS bicscc 612/.01575 Bonner, Philip, author. Protein Purification / by Philip Bonner. Second edition. Boca Raton, FL : Taylor & Francis, [2018]. ©2019. 1 online resource (287 pages) : 125 illustrations, text file, PDF. text rdacontent computer rdamedia online resource rdacarrier THE BASICS (Garland Science) Includes bibliographical references and index. 1. Water, pH and buffers. 2. Protein structure and properties. 3. Chromatography and the strategy of protein purification. 4. Groundwork. 5. Non-affinity absorption techniques used to purify proteins. 6. Affinity-based procedures used to purify proteins. 7. Non-absorption techniques for purifying proteins. 8. Methods for monitoring the purity of protein solutions. Appendix 1: The laws of thermodynamics and the Gibbs free energy equation. Appendix 2: Properties of amino acids. Appendix 3: The common units used in biology. Appendix 4: Chromatographic runs. Appendix 5: Determining the concentration of a compound in a solution in parts per million (ppm) Appendix 6: Theanswers totheexercises in the various chapters. Appendix 7: An alphabetical list of chromatography, electrophoresis, filtration equipment and laboratory suppliers. Appendix 8: Buffer tables to prepare at a required pH value. This second edition of Protein Purification provides a guide to the major chromatographic techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic, starting with the properties of water, progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and their applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.Key Features* Guide to the major techniques used in protein purification* Includes flowcharts to help the reader slect the best purification strategy* Contains step-by-step protocols that guide the reader through each technique and its use* Includes exercises and solutions Also available in print format. Proteins Purification. Proteins isolation & purification. Electronic books. Taylor and Francis. Print version: 9781138312470 BASICS (Garland Science). https://www.taylorfrancis.com/books/9780429458187 Click here to view. 91080 91079